Trapping the Kawasaki Disease Agent
An aerosol sampling device was constructed by the IC3 team in 2010 and transported to Tsukuba University in Japan in 2011, where collaborating Japanese scientists at the National Institute for Environmental Science in conjunction with an IC3 engineer, coordinated 4 airplane flights over Japan in March 2011 for the collection of aerosol samples.
The flight path was determined by the IC3 and NIES teams and 4 sterile filter samples of approximately 90 m3 (90,000 liters) of air per filter were collected and sent on dry ice for their analysis at labs at Columbia University and the Max-Planck Institute-Mainz. Aerosol samples were also collected at ground level. Negative control filters were handled in the same manner as the experimental filters but without aerosol collection and were also sent as benchmark. Final analysis were conducted at Columbia University where handling of filters was conducted under BSL2 conditions to avoid contamination of the filters with ambient organisms or nucleic acid. Results will be reported soon, stay alert!